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Resources

How to Pick ES Cell Colonies

1
Add 15µl of trypsin to each well of a 96-well plate.
2
Set a pipette to 1.5µl and use to dislodge the colonies.
3
Transfer picked colony to trypsin and try not to let sit longer than 30 min.
4
Incubate picked colonies for 5 min at 37 to ensure dissociation.
5
Add 60 µl of ES+Lif to trypsinizing colonies, triturate and transfer to feeder plate set up a day in advance with feeders, in 100µl medium per well.