spacer
spacer
spacer
line.small
spacer.gif
 

Resources

mES Cell Culture Cells Reagents

ES Cell Medium

Reagent
Manufacturer
cat #
Price
V (ml)
Fin C
1
DMEM
MILLIPORE
SLM-220-M
10.69
400
 
2
FBS
HYCLONE
 
75
 
3
Pen/Strep 100X
INVITROGEN
15140-122
10
5
 
4
L-glutamine (200mM) 100X
MILLIPORE
TMS-002-C
8
5
 
5
Non-essential AA 100X
MILLIPORE
TMS-001-C
8
5
 
6
Nucleosides 100Xl
MILLIPORE
ES-008-D
8
5
 
7
2-mercaptoethanol
MILLIPORE
60410-4
8
5
 
8
ESGRO (Lif)
CHEMICON
ESG1107
836
0.05
 

 

Mix, filter through 0.2 µm filter (Corning) | Store at 4°C for maximum of 10 days

MEF Medium

Reagent
Manufacturer
cat #
Price
V (ml)
Fin C
1
DMEM
MILLIPORE
SLM-220-M
10.69
450
 
2
FBS
HYCLONE
E.S. Screened
250
50
 
3
Pen/Strep 100X
INVITROGEN
15140-122
10
5
 
4
L-glutamine 100X
MILLIPORE
TMS-002-C
8
5
 
 

 

Other Reagents

Reagent
Manufacturer
cat #
Price
V (ml)
Fin C
1
Trypsin (0.025%):EDTA
MILLIPORE
SM-2005-C
5.53
1ml for 6cm dia. dish
 
2
2X freezing media w/DMSO
MILLIPORE
ES-002-D
47.4
 
3
1X freezing media w/DMSO
MILLIPORE
S-002-D
33.6
 
4
PBS without Ca and Mg
MILLIPORE
BSS-1006-B
7.6
 
5
Ultra pure water with 0.1% gelatin
MILLIPORE
ES-006-B
10.15